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YY1 Nuclear Loading Control Antibody (3P967)

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YY1 Nuclear Loading Control Antibody (3P967)
产品编号 TMAB-01992
别名 δ transcription factor, Yin and yang 1, Transcriptional repressor protein YY1, NF-E1, INO80S, INO80 complex subunit S, Delta transcription factor

YY1 Nuclear Loading Control Antibody (3P967) 是一种 Rabbit 抗体,靶向 YY1 Nuclear Loading Control。YY1 Nuclear Loading Control Antibody (3P967) 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

YY1 Nuclear Loading Control Antibody (3P967)

YY1 Nuclear Loading Control Antibody (3P967)

Rating icon 还可以
YY1 Nuclear Loading Control Antibody (3P967)
产品编号 TMAB-01992 别名 δ transcription factor, Yin and yang 1, Transcriptional repressor protein YY1, NF-E1, INO80S, INO80 complex subunit S, Delta transcription factor

YY1 Nuclear Loading Control Antibody (3P967) 是一种 Rabbit 抗体,靶向 YY1 Nuclear Loading Control。YY1 Nuclear Loading Control Antibody (3P967) 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

规格价格库存数量
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
YY1 Nuclear Loading Control Antibody (3P967) is a Rabbit antibody targeting YY1 Nuclear Loading Control. YY1 Nuclear Loading Control Antibody (3P967) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
别名δ transcription factor, Yin and yang 1, Transcriptional repressor protein YY1, NF-E1, INO80S, INO80 complex subunit S, Delta transcription factor
Ig Type
IgG
克隆号
3P967
交叉反应
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Human smooth muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
13. Blank control: K562. Primary Antibody (green line): Rabbit Anti-YY1 (Nuclear Loading Control) antibody (TMAB-01992)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
14. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Lane 2: Jurkat (Human) Cell Lysate at 30 μg
Lane 3: Du145 (Human) Cell Lysate at 30 μg
Lane 4: U251 (Human) Cell Lysate at 30 μg
Lane 5: Panc-1 (Human) Cell Lysate at 30 μg
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 7: Molt-4 (Human) Cell Lysate at 30 μg
Lane 8: Hela (Human) Cell Lysate at 30 μg
Lane 9: HL60 (Human) Cell Lysate at 30 μg
Lane 10: Urinary bladder (Mouse) Lysate at 40 μg
Lane 11: Testis (Mouse) Lysate at 40 μg
Primary: Anti-YY1 (TMAB-01992) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65-68 kDa
Observed band size: 65 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; FCM: 1ug/Test
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Nucleus matrix. Note=Associated with the nuclear matrix.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景YY1 is a ubiquitously distributed transcription factor belonging to the GLI Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
抗原信息
免疫原
Recombinant Protein: human YY1
抗原种属
Human
基因ID
Uniprot ID
研究领域
Zinc Finger,Other factors,Transcription Factors,YY1,Neurogenesis
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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