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YY1 Nuclear Loading Control Antibody (3P967) 是一种 Rabbit 抗体,靶向 YY1 Nuclear Loading Control。YY1 Nuclear Loading Control Antibody (3P967) 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
YY1 Nuclear Loading Control Antibody (3P967) 是一种 Rabbit 抗体,靶向 YY1 Nuclear Loading Control。YY1 Nuclear Loading Control Antibody (3P967) 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,390 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | YY1 Nuclear Loading Control Antibody (3P967) is a Rabbit antibody targeting YY1 Nuclear Loading Control. YY1 Nuclear Loading Control Antibody (3P967) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
别名 | δ transcription factor, Yin and yang 1, Transcriptional repressor protein YY1, NF-E1, INO80S, INO80 complex subunit S, Delta transcription factor |
Ig Type | IgG |
克隆号 | 3P967 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Human smooth muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (Rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded (rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (YY1 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (TMAB-01992) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 13. Blank control: K562. Primary Antibody (green line): Rabbit Anti-YY1 (Nuclear Loading Control) antibody (TMAB-01992) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 14. Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 μg Lane 2: Jurkat (Human) Cell Lysate at 30 μg Lane 3: Du145 (Human) Cell Lysate at 30 μg Lane 4: U251 (Human) Cell Lysate at 30 μg Lane 5: Panc-1 (Human) Cell Lysate at 30 μg Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 μg Lane 7: Molt-4 (Human) Cell Lysate at 30 μg Lane 8: Hela (Human) Cell Lysate at 30 μg Lane 9: HL60 (Human) Cell Lysate at 30 μg Lane 10: Urinary bladder (Mouse) Lysate at 40 μg Lane 11: Testis (Mouse) Lysate at 40 μg Primary: Anti-YY1 (TMAB-01992) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65-68 kDa Observed band size: 65 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; FCM: 1ug/Test |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
亚细胞定位 | Nucleus matrix. Note=Associated with the nuclear matrix. |
构建方式 | Recombinant Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | YY1 is a ubiquitously distributed transcription factor belonging to the GLI Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. |
免疫原 | Recombinant Protein: human YY1 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Zinc Finger,Other factors,Transcription Factors,YY1,Neurogenesis |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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