Pyruvate Carboxylase-IN-5 (compound 6m) is a selective and permeable inhibitor of pyruvate carboxylase. This mitochondrial enzyme plays a critical role in catalyzing the conversion of pyruvate to oxaloacetate, a process essential for maintaining the steady-state levels of tricarboxylic acid cycle intermediates, which serve as precursors for the synthesis of biomacromolecules, including amino acids, fatty acids, and glucose.