BP Fluor 430 Azide is a water-soluble, green fluorescent probe activated by azide. It engages in copper-catalyzed click reactions (CuAAC) with terminal alkynes, and can also react with strained cyclooctynes in copper-free click chemistry, forming stable triazoles without the need for Cu catalysts or high temperature. This probe exhibits bright, photostable green fluorescence, with a maximum absorption wavelength around 432 nm and maximum emission wavelength near 539 nm. It is water-soluble and maintains stability across a pH range from 4 to 10. The next-generation BP Fluor 430 Picolyl Azide is also applicable for detecting low-abundance alkyne-labeled biomolecules.