BODIPY TR Cadaverine assay [1] involves quantifying autophagy in cells by incubating them with 125 nM of the compound for 10 minutes. After incubation, the cells are washed four times with PBS and lysed using a buffer containing 10 mM Tris-Cl pH 8 and 0.1% Triton X-100. Fluorescence measurements are then taken with a SPECTRAmax fluorimeter (Molecular Devices) at excitation and emission wavelengths of 588 nm and 616 nm, respectively. To normalize fluorescence levels relative to cell number, 0.2 mM ethidium bromide is added, and DNA fluorescence is read at an excitation wavelength of 530 nm and emission wavelength of 590 nm [1].