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Anti-YB1 Antibody (1K676) 是一种 Rabbit 抗体,靶向 YB1。Anti-YB1 Antibody (1K676) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-YB1 Antibody (1K676) 是一种 Rabbit 抗体,靶向 YB1。Anti-YB1 Antibody (1K676) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-YB1 Antibody (1K676) is a Rabbit antibody targeting YB1. Anti-YB1 Antibody (1K676) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | ybx1, YBX 1, Y-box-binding protein 1, YBOX1_HUMAN, Y-box transcription factor, Y-box binding protein 1, YB 1, Q15905, p50, Nuclease-sensitive element-binding protein 1, Nuclease sensitive element binding protein 1, NSEP1, NSEP 1, MGC117250, MGC110976, MGC104858, MDR NF1, Enhancer factor I subunit A, EFI-A, DNA-binding protein B, DNA binding protein B, DBPB, CSDB, CSDA2, CCAAT-binding transcription factor I subunit A, CCAAT binding transcription factor I subunit A, CBF-A, BP 8 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YB1 antibody. Counter stained with hematoxylin. 6. ICC staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining YB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining YB1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Y-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 50 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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