Anti-XBP1 Polyclonal Antibody is a Rabbit antibody targeting XBP1. Anti-XBP1 Polyclonal Antibody can be used in WB,ICC/IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Cow)

1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (XBP1) polyclonal Antibody, Unconjugated (TMAB-01989) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. U-937 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with XBP1 Antibody (TMAB-01989) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
3. Sample:
Lane 1: Mouse Skin tissue lysates
Lane 2: Rat Skin tissue lysates
Lane 3: Rat Testis tissue lysates
Primary: Anti-XBP1 (TMAB-01989) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29/40 kDa
Observed band size: 29/42 kDa

WB: 1:500-2000; ICC/IF: 1:100; FCM: 1ug/test

Polyclonal

KLH conjugated synthetic peptide: human XBP-1

Human

Transcription Factors,Other factors