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Anti-Wnt5a Antibody (2Q540)
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产品编号 TMAC-04288
别名 WNT5A, Protein Wnt-5a
Anti-Wnt5a Antibody (2Q540) 是一种 Rabbit 抗体，靶向 Wnt5a。Anti-Wnt5a Antibody (2Q540) 可用于 WB,ICC,IHC,FCM。
Anti-Wnt5a Antibody (2Q540)
Anti-Wnt5a Antibody (2Q540)
还可以产品编号 TMAC-04288 别名 WNT5A, Protein Wnt-5a
Anti-Wnt5a Antibody (2Q540) 是一种 Rabbit 抗体，靶向 Wnt5a。Anti-Wnt5a Antibody (2Q540) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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| 产品描述 | Anti-Wnt5a Antibody (2Q540) is a Rabbit antibody targeting Wnt5a. Anti-Wnt5a Antibody (2Q540) can be used in WB,ICC,IHC,FCM. |
| 别名 | WNT5A, Protein Wnt-5a |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Wnt5a on Hela cell lysate using anti-Wnt5a antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Wnt5a antibody. Counter stained with hematoxylin. 3. ICC staining Wnt5a in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of SH-SY5Y cells with Wnt5a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene "wingless" and to its vertebrate ortholog, Int1, a mouse protooncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Northern blot analysis detects expression of Wnt-5a in brain, lung, and heart. At least five distinct Wnt-5a transcripts are observed, which are due to transcript variability 5-prime to the initiation methionine. In situ hybridization detects a complex spatial and temporal pattern of Wnt-5a in the mouse, including expression in the developing central nervous system, limbs, facial processes, and the posterior region of the fetus. Human frizzled-5 is the receptor for the Wnt-5a ligand. It is suggested that Wnt-5a augments primitive hematopoietic development in vivo and represents an in vivo regulator of hematopoietic stem cell function in the human. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 42 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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