Anti-Wilms tumor/WT1 Antibody (8L145) is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (8L145) can be used in WB,ICC/IF,IHC,FCM.

IgG

Human,Mouse

1. ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
2. ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
5. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
6. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
7. Western blot analysis of WT1 expression in K562 cell lysate.
8. Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.

WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200; FCM: 1:20-100

Monoclonal

Unconjugated

A synthesized peptide: human WT1

human