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Anti-VPS35 Antibody (7F247)
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产品编号 TMAC-04270
别名 VPS35, Vesicle protein sorting 35, Vacuolar protein sorting-associated protein 35, MEM3, Maternal-embryonic 3, hVPS35
Anti-VPS35 Antibody (7F247) 是一种 Rabbit 抗体，靶向 VPS35。Anti-VPS35 Antibody (7F247) 可用于 WB,IHC,FCM。
Anti-VPS35 Antibody (7F247)
Anti-VPS35 Antibody (7F247)
还可以产品编号 TMAC-04270 别名 VPS35, Vesicle protein sorting 35, Vacuolar protein sorting-associated protein 35, MEM3, Maternal-embryonic 3, hVPS35
Anti-VPS35 Antibody (7F247) 是一种 Rabbit 抗体，靶向 VPS35。Anti-VPS35 Antibody (7F247) 可用于 WB,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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| 产品描述 | Anti-VPS35 Antibody (7F247) is a Rabbit antibody targeting VPS35. Anti-VPS35 Antibody (7F247) can be used in WB,IHC,FCM. |
| 别名 | VPS35, Vesicle protein sorting 35, Vacuolar protein sorting-associated protein 35, MEM3, Maternal-embryonic 3, hVPS35 |
| Ig Type | IgG |
| 克隆号 | 7F247 |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M, Lane 2: U937, Lane 3: SH-SY-5Y. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:100-300; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS35 (vacuolar protein sorting 35), also known as MEM3, is the 796 amino acid human homolog of the S. cerevisiae Vps35 protein. Localized to the cytoplasm and to the peripheral membrane, VPS35 is an essential component of the retromer complex which is involved in retrieval of lysosomal enzyme receptors from endosomes to the trans-Golgi network. VPS35 is expressed ubiquitously with highest expression in heart, placenta, brain, testis, kidney, colon, ovary and spleen. In addition to its crucial role in the retromer complex, VPS35 is part of a subcomplex that is required to regulate transcytosis of the polymeric immunoglobulin receptor from the basolateral to the apical surface of epithelial cells and hepatocytes. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 91 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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