Anti-VEGFC Polyclonal Antibody is a Rabbit antibody targeting VEGFC. Anti-VEGFC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (VEGF-C) Polyclonal Antibody, Unconjugated (TMAB-01954) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-VEGF-C antibody (TMAB-01954)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-VEGF-C antibody (TMAB-01954) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Sample:
Lane 1: LympHnode (Mouse) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: Thymus (Rat) Lysate at 40 μg
Lane 4: LympHnode (Rat) Lysate at 40 μg
Lane 5: Kidney (Rat) Lysate at 40 μg
Primary:
Anti-VEGF-C (TMAB-01954) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human VEGF-C

Human

VEGF,Angiogenic growth factors,VEGF,Neurogenesis,VEGF