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Anti-VEGFA Antibody (8D813) 是一种 Rabbit 抗体,靶向 VEGFA。Anti-VEGFA Antibody (8D813) 可用于 ICC/IF,IHC,IP,FCM。
Anti-VEGFA Antibody (8D813) 是一种 Rabbit 抗体,靶向 VEGFA。Anti-VEGFA Antibody (8D813) 可用于 ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-VEGFA Antibody (8D813) is a Rabbit antibody targeting VEGFA. Anti-VEGFA Antibody (8D813) can be used in ICC/IF,IHC,IP,FCM. |
别名 | 血管内皮生长因子 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VEGF antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human breast carcimoma tissue using anti-VEGF antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGF antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-VEGF antibody. Counter stained with hematoxylin. 6. ICC staining VEGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining VEGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining VEGF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining VEGF in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with VEGF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 43 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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