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顶部生物活性储存和溶解信息计算器

Anti-VAMP3 Antibody (5C283)

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产品编号 TMAC-00812

别名 vesicle-associated membrane protein 3, CEB

Anti-VAMP3 Antibody (5C283) 是一种 Rabbit 抗体，靶向 VAMP3。Anti-VAMP3 Antibody (5C283) 可用于 FCM,ICC,IF,IHC,WB。

Anti-VAMP3 Antibody (5C283)

Anti-VAMP3 Antibody (5C283)

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产品编号 TMAC-00812 别名 vesicle-associated membrane protein 3, CEB

Anti-VAMP3 Antibody (5C283) 是一种 Rabbit 抗体，靶向 VAMP3。Anti-VAMP3 Antibody (5C283) 可用于 FCM,ICC,IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-VAMP3 Antibody (5C283) is a Rabbit antibody targeting VAMP3. Anti-VAMP3 Antibody (5C283) can be used in FCM,ICC,IF,IHC,WB.
别名vesicle-associated membrane protein 3, CEB
Ig Type
IgG
克隆号
5C283
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Cellubrevin on A549 (1), 293T(2) cell lysates using anti-Cellubrevin antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
5. ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Cellubrevin in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用FCMICCIFIHCWB
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Vesicle-associated membrane proteins, known as VAMPs, also designated synaptobrevins, include VAMP-1, VAMP-2, VAMP-3 (cellubrevin), and synaptotagmin, a protein that may function as an inhibitor of exocytosis. VAMP proteins are vesicular factors that are important components of the machinery controlling docking and/or fusion of secretory vesicles with their target membrane. Synaptosomal-associated proteins, known as SNAPs, including alpha- and gamma-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. Pancreatic beta-cells express VAMP-2 and VAMP-3, and either one or both of these proteins selectively control Ca2+-mediated insulin secretion. In addition, VAMP-2 and VAMP-3 are expressed on GLUT4-containing vesicle membranes isolated from 3T3-Ll adipocytes and are important components of the insulin-dependent translocation of GLUT4 to the cell surface in adipocytes.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
KLH conjugated synthetic peptide: within N-terminal human Cellubrevin
抗原种属
human
Uniprot ID
Q15836
化学信息
分子量Theoretical: 11 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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