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Anti-VAMP2 Antibody (1D488)
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产品编号 TMAC-04216
别名 Vesicle-associated membrane protein 2, VAMP-2, Vamp2, Synaptobrevin-2, Syb2
Anti-VAMP2 Antibody (1D488) 是一种 Rabbit 抗体，靶向 VAMP2。Anti-VAMP2 Antibody (1D488) 可用于 WB,IP,ICC/IF,FCM。
Anti-VAMP2 Antibody (1D488)
Anti-VAMP2 Antibody (1D488)
还可以产品编号 TMAC-04216 别名 Vesicle-associated membrane protein 2, VAMP-2, Vamp2, Synaptobrevin-2, Syb2
Anti-VAMP2 Antibody (1D488) 是一种 Rabbit 抗体，靶向 VAMP2。Anti-VAMP2 Antibody (1D488) 可用于 WB,IP,ICC/IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-VAMP2 Antibody (1D488) is a Rabbit antibody targeting VAMP2. Anti-VAMP2 Antibody (1D488) can be used in WB,IP,ICC/IF,FCM. |
| 别名 | Vesicle-associated membrane protein 2, VAMP-2, Vamp2, Synaptobrevin-2, Syb2 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY5Y, Lane 2: Jurkat. 2. ICC staining VAMP2 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining VAMP2 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining VAMP2 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY5Y cells with VAMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and γ-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exo-cytosis by competing with SNAP 25 and syntaxins for VAMP binding. |
| 偶联 | Unconjugated |
| 免疫原 | A synthesized peptide: within Human VAMP2 aa 9-42 / 116 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 15 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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