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顶部产品介绍偶联与修饰储存和溶解信息计算器

Anti-V5 tag Antibody (9T763)

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货号 TMAS-00145

别名 V5tag

Anti-V5 tag Antibody (9T763) 是一种 Mouse 抗体，靶向 V5 tag。Anti-V5 tag Antibody (9T763) 可用于 ELISA, FCM, ICC/IF, IP, WB。

Anti-V5 tag Antibody (9T763)

Anti-V5 tag Antibody (9T763)

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货号 TMAS-00145 别名 V5tag

Anti-V5 tag Antibody (9T763) 是一种 Mouse 抗体，靶向 V5 tag。Anti-V5 tag Antibody (9T763) 可用于 ELISA, FCM, ICC/IF, IP, WB。

规格价格库存数量
100 μg
¥ 2,925
5日内发货
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产品介绍

生物活性
产品描述
Anti-V5 tag Antibody (9T763) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (9T763) can be used in ELISA, FCM, ICC/IF, IP, WB.
别名V5tag
Ig Type
IgG2a,κ
克隆号
9T763
特异性
C-terminal, N-terminal, and internal V5 tagged fusion proteins
验证活性
1. Sensitivity comparison of purified internal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG
2. Immunocytochemistry/Immunofluenrescence analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
3. Comparison sensitivity of purified N-terminal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG
4. Flow cytometric analysis of non-transfected or V5 fusion gene transfected CHO cells using Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
5. Flow cytometric analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
6. Western blot analysis of immunoprecipitates from V5-tagged protein transfected HEK293 cell lysates using Anti-V5 tag Antibody (9T763) (TMAS-00145).
Lane 1: V5-tagged protein transfected HEK293 cell lysate as control.
Lane 2-3: Immunoprecipitates of V5-tagged protein transfected and non-transfected HEK293 cell lysate with Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.
7. Sensitivity comparison of purified internal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.
8. Superior specificity assay of transfected cell lysates using Western blot with Anti-V5 tag Antibody (9T763) (TMAS-00145).
Lane 1: V5-tagged protein transfected CHO cell lysate.
Lane 2: Non-transfected CHO cell lysate.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.
应用ELISAFCMICC/IFIPWB
推荐剂量
ELISA: 0.05-0.1 µg/ml; FCM: 1-3 µg for 1 x 10 6 cells; ICC/IF: 1-3 µg/ml; IP: 1-5 µg/ml; WB: 0.2-1 µg/ml
抗体种类
Monoclonal
宿主来源Mouse
纯化方式Protein A affinity column
性状Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
缓冲液Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
研究背景The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
偶联与修饰
偶联
Unconjugated
其他可选修饰符
HRP/Biotin/iFluor 488/iFluor 555/iFluor 647
可提供抗体种类
6
抗原信息
免疫原
KLH conjugated synthetic peptide: GKPIPNPLLGLDST
储存&溶解度
储存方式The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储-20°C for 1 year

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