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Anti-USP7 Antibody (7H195) 是一种 Mouse 抗体，靶向 USP7。Anti-USP7 Antibody (7H195) 可用于 ELISA,FCM。
还可以Anti-USP7 Antibody (7H195) 是一种 Mouse 抗体，靶向 USP7。Anti-USP7 Antibody (7H195) 可用于 ELISA,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,679 | 5日内发货 |
| 产品描述 | Anti-USP7 Antibody (7H195) is a Mouse antibody targeting USP7. Anti-USP7 Antibody (7H195) can be used in ELISA,FCM. |
| 别名 | ubiquitin specific peptidase 7 (herpes virus-associated), TEF1, HAUSP |
| Ig Type | Mouse IgG2a |
| 克隆号 | 7H195 |
| 交叉反应 | Human |
| 验证活性 | Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA,FCM |
| 推荐剂量 | ELISA: 1:1000-1:2000; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human USP7 / HAUSP (rh USP7 / HAUSP; TMPY-01463; NP_003461.2; Lys208-Glu560). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Ubiquitin carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein that belongs to the peptidase C19 family. USP7 is a member of the family of deubiquitinating enzymes. It is involved in the regulation of stress response pathways, epigenetic silencing and the progress of infections by DNA viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal domains required for protein-protein interactions. USP7 contributes to epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin fusion protein substrates. It deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2. USP7 also induces TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type 1 (HSV-1) regulatory protein ICP stimulates lytic infection and the reactivation of quiescent viral genomes. ICP interacts very strongly with USP7. USP7-mediated stabilization of ICP is dominant over ICP-induced degradation of USP7 during productive HSV-1 infection. The biological significance of the ICP-USP7 interaction may be most pronounced in natural infection situations, in which limited amounts of ICP are expressed. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human USP7 / HAUSP protein (TMPY-01463) |
| 抗原种属 | Human |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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