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Anti-USP22 Antibody (6P785) 是一种抗体,靶向 USP22。Anti-USP22 Antibody (6P785) 可用于 ELISA, IF, FC。
Anti-USP22 Antibody (6P785) 是一种抗体,靶向 USP22。Anti-USP22 Antibody (6P785) 可用于 ELISA, IF, FC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,300 | 5日内发货 | |
100 μL | ¥ 2,190 | 5日内发货 |
产品描述 | Anti-USP22 Antibody (6P785) is an antibody targeting USP22. Anti-USP22 Antibody (6P785) can be used in ELISA, IF, FC. |
别名 | USP3L, USP 22, Ubiquitin-specific-processing protease 22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, EC 3.4.19.12, Deubiquitinating enzyme 22 |
Ig Type | Rabbit IgG |
克隆号 | 6P785 |
交叉反应 | Human |
验证活性 | 1. Immunofluorescence staining of MCF-7 with TMAH-01238 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 493-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing Hela cells stained with TMAH-01238 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
应用 | ELISA, IF, FC |
推荐剂量 | IF:1:50-1:200; FC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Nucleus. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human USP22 |
抗原种属 | Human |
基因ID | 23326 |
Uniprot ID | |
研究领域 | Epigenetics and Nuclear Signaling, Cell biology |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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