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Anti-USP13 Antibody (7A655) 是一种 Rabbit 抗体,靶向 USP13。Anti-USP13 Antibody (7A655) 可用于 WB,ICC/IF,FCM,IP。
Anti-USP13 Antibody (7A655) 是一种 Rabbit 抗体,靶向 USP13。Anti-USP13 Antibody (7A655) 可用于 WB,ICC/IF,FCM,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-USP13 Antibody (7A655) is a Rabbit antibody targeting USP13. Anti-USP13 Antibody (7A655) can be used in WB,ICC/IF,FCM,IP. |
别名 | USP 13, UBP13_HUMAN, UBP13, Ubiquitin-specific-processing protease 13, Ubiquitin thiolesterase 13, Ubiquitin thioesterase 13, Ubiquitin specific protease 13, Ubiquitin specific processing protease 13, Ubiquitin specific peptidase 13 (isopeptidase T3), Ubiquitin specific peptidase 13, Ubiquitin carboxyl-terminal hydrolase 13, Ubiquitin carboxyl terminal hydrolase 13, ISOT-3, ISOT3, ISOT 3, Isopeptidase T-3, Isopeptidase T3, Isopeptidase T 3, Deubiquitinating enzyme 13 |
Ig Type | IgG |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of USP13 on mouse testis tissue lysate using anti-USP13 antibody at 1/500 dilution. 2. ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining USP13 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining USP13 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY5Y cells with USP13 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
应用 | |
推荐剂量 | WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 97 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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