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顶部生物活性储存和溶解信息计算器

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)

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Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)
产品编号 TMAC-04193
别名 ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) 是一种 Rabbit 抗体，靶向 Ubiquitin Activating Enzyme E1/UBA1。Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) 可用于 WB,ICC/IF,IHC,FCM。

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)

Rating icon 还可以
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154)
产品编号 TMAC-04193 别名 ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9

Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) 是一种 Rabbit 抗体，靶向 Ubiquitin Activating Enzyme E1/UBA1。Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,495
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100 μL
¥ 2,480
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产品介绍

生物活性
产品描述
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) is a Rabbit antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1V154) can be used in WB,ICC/IF,IHC,FCM.
别名ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
4. ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining UBA1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining UBA1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of K562 cells with UBA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
FCM
推荐剂量
WB: 1:1000-2000; IHC: 1:100-500; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. Specifically, UBE1 functions to adenylate the C-terminal glycine residue of ubiquitin, a reaction that is ATP-dependent and is proceeded by the formation of a thiolester bond with a cysteine residue of UBE1. The UBE1-activated ubiquitin is then transferred to a ubiquitin conjugated enzyme, which donates the ubiquitin residue to target substrates. The UBE1 gene is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. However, no UBE1 homolog is detectable on the human Y chromosome. UBE1 is thought to escape X inactivation in humans.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P22314
化学信息
分子量Theoretical: 118 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

UBE-1UBE 1SMAX-2SMAX 2POC-20POC 20GXP-1GXP 1CFAP-124CFAP 124AMCX-1AMCX 1

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