Anti-UBC Antibody (1V28)

Name: Anti-UBC Antibody (1V28)
Brand: TargetMol
SKU: TMAH-01232
Rating: 4.5 (1 reviews)

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产品编号 TMAH-01232

别名 UBC, Polyubiquitin-C [Cleaved into: Ubiquitin]

Anti-UBC Antibody (1V28) 是一种抗体，靶向 UBC。Anti-UBC Antibody (1V28) 可用于 ELISA, FCM。

Anti-UBC Antibody (1V28)

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产品编号 TMAH-01232 别名 UBC, Polyubiquitin-C [Cleaved into: Ubiquitin]

Anti-UBC Antibody (1V28) 是一种抗体，靶向 UBC。Anti-UBC Antibody (1V28) 可用于 ELISA, FCM。

规格	价格	库存	数量
50 μL	¥ 1,305	5日内发货
100 μL	¥ 2,180	5日内发货

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产品描述	Anti-UBC Antibody (1V28) is an antibody targeting UBC. Anti-UBC Antibody (1V28) can be used in ELISA, FCM.
别名	UBC, Polyubiquitin-C [Cleaved into: Ubiquitin]
Ig Type	Mouse IgG2a
克隆号	1V28
交叉反应	Human
验证活性	Overlay Peak curve showing MCF7 cells stained with TMAH-01232 (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/110^6 cells) used under the same conditions. Acquisition of >10, 022 events was performed.
应用	ELISA, FCM
推荐剂量	FCM:1:20-1:500.
抗体种类	Monoclonal
亚细胞定位	[Ubiquitin]: Cytoplasm. Nucleus.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
研究背景	Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Protein: Human UBC Protein
抗原种属	Human
基因ID	7316
Uniprot ID	P0CG48
研究领域	Epigenetics and Nuclear Signaling, Neuroscience, Cell biology