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Anti-TUBB Antibody (8X869) 是一种 Mouse 抗体,靶向 TUBB。Anti-TUBB Antibody (8X869) 可用于 WB,ICC,IHC,FCM。
Anti-TUBB Antibody (8X869) 是一种 Mouse 抗体,靶向 TUBB。Anti-TUBB Antibody (8X869) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,995 | 5日内发货 | |
100 μL | ¥ 2,980 | 5日内发货 |
产品描述 | Anti-TUBB Antibody (8X869) is a Mouse antibody targeting TUBB. Anti-TUBB Antibody (8X869) can be used in WB,ICC,IHC,FCM. |
别名 | Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB |
交叉反应 | Human,Mouse,Rat,zebrafish |
验证活性 | 1. Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution. Positive control: Lane 1: NCCIT, Lane 2: NIH/3T3, Lane 3: PC12, Lane 4: Mouse heart, Lane 5: F9, Lane 6: zebrafish brain, Lane 7: Hela. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin. 3. ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining:-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of HeLa?cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:5000-10000; IHC: 1:20; ICC: 1:200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP. |
偶联 | Unconjugated |
免疫原 | Peptide |
Uniprot ID |
分子量 | Theoretical: 50 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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