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Anti-TSG101 Antibody (9U262)
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产品编号 TMAC-04144
Anti-TSG101 Antibody (9U262) 是一种 Rabbit 抗体，靶向 TSG101。Anti-TSG101 Antibody (9U262) 可用于 WB,ICC/IF,IHC,FCM。
Anti-TSG101 Antibody (9U262)
Anti-TSG101 Antibody (9U262)
还可以产品编号 TMAC-04144
Anti-TSG101 Antibody (9U262) 是一种 Rabbit 抗体，靶向 TSG101。Anti-TSG101 Antibody (9U262) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-TSG101 Antibody (9U262) is a Rabbit antibody targeting TSG101. Anti-TSG101 Antibody (9U262) can be used in WB,ICC/IF,IHC,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of TSG101 on different lysates using anti-TSG101 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3, Lane 2: Hela, Lane 3: K562, Lane 4: Jurkat, Lane 5: A431. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin. 6. ICC staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 44 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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