Anti-TREM-1 Polyclonal Antibody is a Rabbit antibody targeting TREM-1. Anti-TREM-1 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Rat

1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (TMAB-01891) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Primary Antibody: Rabbit anti-TREM1 antibody Supplier catalog number: TMAB-01891 Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: f (ab/)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature)
3. Sample:
hcclm3 (Human) Cell Lysate at 30 μg
K562 (Human) Cell Lysate at 30 μg
Primary: Anti-TREM1 (TMAB-01891) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 21 kDa
4. Primary Antibody: Rabbit anti-TREM1 antibody
Supplier catalog number: TMAB-01891
Supplier: Bioss
Isotype Control Antibody: Rabbit IgG
Secondary Antibody: f (ab/)2 fragment goat anti-rabbit IgG-PE
Supplier: Jackson Lab.
Positive control: U937 (4% paraformaldehyde for 10 min at room temperature)

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human TREM1

Human

TREM1

Triggering receptor expressed on myeloid cells 1

Macrophage / Inflamm.

Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity).