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Anti-TREM-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TREM-1。Anti-TREM-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
Anti-TREM-1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TREM-1。Anti-TREM-1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-TREM-1 Polyclonal Antibody is a Rabbit antibody targeting TREM-1. Anti-TREM-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
交叉反应 | Human,Rat |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (TMAB-01891) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Primary Antibody: Rabbit anti-TREM1 antibody Supplier catalog number: TMAB-01891 Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: f (ab/)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature) 3. Sample: hcclm3 (Human) Cell Lysate at 30 μg K562 (Human) Cell Lysate at 30 μg Primary: Anti-TREM1 (TMAB-01891) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 21 kDa 4. Primary Antibody: Rabbit anti-TREM1 antibody Supplier catalog number: TMAB-01891 Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: f (ab/)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control: U937 (4% paraformaldehyde for 10 min at room temperature) |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]. |
免疫原 | KLH conjugated synthetic peptide: human TREM1 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Macrophage / Inflamm. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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