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Anti-Transferrin Receptor/TFRC Antibody (6G357)

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产品编号 TMAY-01475

Anti-Transferrin Receptor/TFRC Antibody (6G357) 是一种 Mouse 抗体,靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (6G357) 可用于 FCM,ICC/IF。

Anti-Transferrin Receptor/TFRC Antibody (6G357)

Anti-Transferrin Receptor/TFRC Antibody (6G357)

Rating icon 还可以
产品编号 TMAY-01475

Anti-Transferrin Receptor/TFRC Antibody (6G357) 是一种 Mouse 抗体,靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (6G357) 可用于 FCM,ICC/IF。

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产品介绍

生物活性
产品描述
Anti-Transferrin Receptor/TFRC Antibody (6G357) is a Mouse antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (6G357) can be used in FCM,ICC/IF.
Ig Type
Monoclonal Mouse IgG1
克隆号
6G357
交叉反应
Human
验证活性
1. Flow cytometric analysis of Human TFRC(CD71) expression on U937 cells. Cells were stained with purified anti-Human TFRC(CD71), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

2. Immunofluorescence staining of CD71 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human CD71 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
应用
FCM,ICC/IF
推荐剂量
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; TMPY-01873; AAA61153.1; Cys89-Phe760). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
APC/PerCP/FITC/PE
可提供抗体种类
5
抗原信息
免疫原
Recombinant Human Transferrin Receptor / TFRC / CD71 Protein (TMPY-01873)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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