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Anti-Transferrin Receptor/TFRC Antibody (5P550)
还可以产品编号 TMAY-01478
Anti-Transferrin Receptor/TFRC Antibody (5P550) 是一种 Rabbit 抗体，靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (5P550) 可用于 FCM,ICC/IF。
Anti-Transferrin Receptor/TFRC Antibody (5P550)
Anti-Transferrin Receptor/TFRC Antibody (5P550)
还可以产品编号 TMAY-01478
Anti-Transferrin Receptor/TFRC Antibody (5P550) 是一种 Rabbit 抗体，靶向 Transferrin Receptor/TFRC。Anti-Transferrin Receptor/TFRC Antibody (5P550) 可用于 FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Transferrin Receptor/TFRC Antibody (5P550) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (5P550) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5P550 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human TFRC in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TFRC monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human TFRC(CD71) expression on U937 cells. Cells were stained with purified anti-Human TFRC(CD71), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; TMPY-01873; AAA61153.1; Cys89-Phe760). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PerCP/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Human Transferrin Receptor / TFRC / CD71 protein (TMPY-01873) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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