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Anti-TRAF2 Antibody (6B229) 是一种 Rabbit 抗体,靶向 TRAF2。Anti-TRAF2 Antibody (6B229) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-TRAF2 Antibody (6B229) 是一种 Rabbit 抗体,靶向 TRAF2。Anti-TRAF2 Antibody (6B229) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-TRAF2 Antibody (6B229) is a Rabbit antibody targeting TRAF2. Anti-TRAF2 Antibody (6B229) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | Tumor necrosis factor type 2 receptor-associated protein 3, TRAP3, TRAF 2, TNF receptor-associated factor 2, RING-type E3 ubiquitin transferase TRAF2, EC 2.3.2.27, E3 ubiquitin-protein ligase TRAF2 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of TRAF2 on Hela cells lysates using anti-TRAF2 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TRAF2 antibody. Counter stained with hematoxylin. 6. ICC staining TRAF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining TRAF2 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining TRAF2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with TRAF2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily, which includes the FAS antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a ??TRAF-C?? domain that is necessary and sufficient for CD40 binding and homodimerization. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 56 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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