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Anti-TPSB2 Antibody (4C309) 是一种抗体,靶向 TPSB2。Anti-TPSB2 Antibody (4C309) 可用于 ELISA, WB, FCM。
Anti-TPSB2 Antibody (4C309) 是一种抗体,靶向 TPSB2。Anti-TPSB2 Antibody (4C309) 可用于 ELISA, WB, FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,305 | 5日内发货 | |
100 μL | ¥ 2,195 | 5日内发货 |
产品描述 | Anti-TPSB2 Antibody (4C309) is an antibody targeting TPSB2. Anti-TPSB2 Antibody (4C309) can be used in ELISA, WB, FCM. |
Ig Type | Rabbit IgG |
克隆号 | 4C309 |
交叉反应 | Human, Mouse |
验证活性 | 1. Western Blot -Positive WB detected in: Mouse Kidney tissue lysate, -All lanes: TPSB2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 30 kDa -Observed band size: 30 kDa 2. Overlay Peak curve showing K562 cells stained with TMAH-01210 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
应用 | ELISA, WB, FCM |
推荐剂量 | WB:1:500-1:2000; FCM:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Secreted. Note=Released from the secretory granules upon mast cell activation. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human TPSB2 |
抗原种属 | Human |
基因ID | 64499 |
Uniprot ID | |
研究领域 | Others |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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