Anti-TNFR2/CD120b/TNFR1B Antibody (1J191) is a Rabbit antibody targeting TNFR2/CD120b/TNFR1B. Anti-TNFR2/CD120b/TNFR1B Antibody (1J191) can be used in IF,IHC-Fr,IHC-P.

IgG

1J191

Human (predicted:Mouse,Rat)

1. Sample:
Lane 1: Human THP-1 cell Lysates
Lane 2: Human K562 cell Lysates
Lane 3: Human U937 cell Lysates
Lane 4: Human U87MG cell Lysates
Lane 5: Human HUVEC cell Lysates
Primary: Anti-TNFR2 (TMAB-01874) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 70 kDa
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (TMAB-01874) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (TMAB-01874) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNFR2 Monoclonal Antibody, Unconjugated (TMAB-01874) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human TNFRSF1B

Human

TNFRSF1B

Tumor necrosis factor receptor superfamily member 1B

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.