Anti-TFRC Antibody (4O872) is a Mouse antibody targeting TFRC. Anti-TFRC Antibody (4O872) can be used in WB,ICC/IF,FCM.

IgG1

4O872

Human

1. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HT-1080 cell lysates
Primary: Anti-TFRC (TMAB-13482) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 74/85 kDa
Observed band size: 95 kDa
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (TMAB-13482) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
3. Blank control: Hela.
Primary Antibody (green line): Mouse Anti-TFRC antibody (TMAB-13482)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 2μg/Test

Monoclonal

Recombinant Protein: human CD71 protein

Human

TFRC

Transferrin receptor protein 1

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.