Anti-TAZ Polyclonal Antibody is a Rabbit antibody targeting TAZ. Anti-TAZ Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (TMAB-01810) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (TMAB-01810) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
MCF-7 (Human) Cell Lysate at 40 μg
LOVO (Human) CellLysate at 40 μg
A549 (Human) CellLysate at 40 μg
Primary: Anti-TAz (TMAB-01810) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 46 kDa
4. Blank control: A549. Primary Antibody (green line): Rabbit Anti-TAZ antibody (TMAB-01810) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 3 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Kidney (Rat) Lysate at 40 μg
Primary:
Anti-TAZ (TMAB-01810) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kDa
Observed band size: 52 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3ug/test

Polyclonal

KLH conjugated synthetic peptide: human TAZ

Human