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Anti-Syndecan-4 Antibody (7T56)

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产品编号 TMAY-03438

Anti-Syndecan-4 Antibody (7T56) 是一种 Rabbit 抗体,靶向 Syndecan-4。Anti-Syndecan-4 Antibody (7T56) 可用于 WB,FCM。

Anti-Syndecan-4 Antibody (7T56)

Anti-Syndecan-4 Antibody (7T56)

Rating icon 还可以
产品编号 TMAY-03438

Anti-Syndecan-4 Antibody (7T56) 是一种 Rabbit 抗体,靶向 Syndecan-4。Anti-Syndecan-4 Antibody (7T56) 可用于 WB,FCM。

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产品介绍

生物活性
产品描述
Anti-Syndecan-4 Antibody (7T56) is a Rabbit antibody targeting Syndecan-4. Anti-Syndecan-4 Antibody (7T56) can be used in WB,FCM.
Ig Type
Monoclonal Rabbit IgG
克隆号
7T56
交叉反应
Mouse
验证活性
1. Anti-SDC4 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:22 kDa.

-Observed band size:22 kDa.

2. Flow cytometric analysis of Mouse SDC4 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SDC4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
应用
WB,FCM
推荐剂量
WB: 1:500-1:1000; FCM: 1:25-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Syndecan-4 / SDC4 (rM Syndecan-4 / SDC4; TMPY-02392; O35988; Met1-Val146).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景SDC4 (Syndecan-4), also known as Syn4, is a transmembrane heparan sulfate proteoglycan that co-operates with integrins during cell-matrix interactions for the assembly of focal adhesions and actin stress fibers and in the phosphorylation of focal adhesion kinase (FAK) on Tyr397. Syndecan-4 plays roles in the formation of focal adhesions and stress fibers. The cytoplasmic domain of syndecan-4 interacts with several signalling and structural proteins, and both extracellular and cytoplasmic domains are necessary for regulated activation of associated transmembrane receptors. Syndecan-4/SDC4 is a heparan sulfate proteoglycan and works as a coreceptor for various growth factors. SDC4 deficiency limits neointimal formation after vascular injury by regulating vascular smooth muscle cells (VSMCs) proliferation and vascular progenitor cells (VPCs) mobilization. Therefore, SDC4 may be a novel therapeutic target for preventing arterial restenosis after angioplasty.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
PE
可提供抗体种类
2
抗原信息
免疫原
Recombinant Mouse Syndecan-4 / SDC4 protein (TMPY-02392)
抗原种属
Mouse
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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