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Anti-SUMO tag Antibody (5Y461) 是一种 Mouse 抗体,靶向 SUMO tag。Anti-SUMO tag Antibody (5Y461) 可用于 ELISA, WB。
Anti-SUMO tag Antibody (5Y461) 是一种 Mouse 抗体,靶向 SUMO tag。Anti-SUMO tag Antibody (5Y461) 可用于 ELISA, WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 40 μg | ¥ 1,609 | 5日内发货 |
| 产品描述 | Anti-SUMO tag Antibody (5Y461) is a Mouse antibody targeting SUMO tag. Anti-SUMO tag Antibody (5Y461) can be used in ELISA, WB. |
| Ig Type | IgG2a,κ |
| 克隆号 | 5Y461 |
| 特异性 | Fusion proteins containing SUMO epitope tags |
| 验证活性 | 1. Western Blot of SUMO protein with Anti-SUMO tag Antibody (5Y461) (TMAS-00131). Lane 1: 20 ng SUMO protein Lane 2: 10 ng SUMO protein Lane 3: 5 ng SUMO protein Primary Antibody: Anti-SUMO tag Antibody (5Y461) (TMAS-00131), 1 µg/ml. Secondary Antibody: Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Fragment Specific, 0.2 μg/ml Predicted Size: 11 kD Observed Size: 18 kD 2. Western blot analysis of SUMO-tagged fusion protein using Anti-SUMO tag Antibody (5Y461) (TMAS-00131). The signal was developed with IRDyeTM800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 62 kD Observed Size: 62 kD |
| 应用 | ELISAWB |
| 推荐剂量 | ELISA: 0.05-0.2 µg/ml; WB: 0.5-1 µg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 纯化方式 | Protein A affinity column |
| 性状 | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| 缓冲液 | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| 研究背景 | SUMO tags are emerging as a viable alternative for increasing both the expression and solubility of otherwise hard-to-express proteins. The SUMO tag can be cleanly excised using SUMO protease, which recognizes the conformation of SUMO protein rather than a specific sequence within SUMO. The SUMO system can be used effectively in both prokaryotic and eukaryotic systems. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: yeast SUMO protein (DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDG IRIQADQAPEDLDMEDNDIIEAHREQIGG) |
| 储存方式 | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | -20°C for 1 year |