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Anti-SUMO tag Antibody (5Y461)

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货号 TMAS-00131

Anti-SUMO tag Antibody (5Y461) 是一种 Mouse 抗体,靶向 SUMO tag。Anti-SUMO tag Antibody (5Y461) 可用于 ELISA, WB。

Anti-SUMO tag Antibody (5Y461)

Anti-SUMO tag Antibody (5Y461)

一键复制产品信息
Rating icon 还可以
货号 TMAS-00131

Anti-SUMO tag Antibody (5Y461) 是一种 Mouse 抗体,靶向 SUMO tag。Anti-SUMO tag Antibody (5Y461) 可用于 ELISA, WB。

规格价格库存数量
40 μg
¥ 1,609
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产品介绍


生物活性
产品描述
Anti-SUMO tag Antibody (5Y461) is a Mouse antibody targeting SUMO tag. Anti-SUMO tag Antibody (5Y461) can be used in ELISA, WB.
Ig Type
IgG2a,κ
克隆号
5Y461
特异性
Fusion proteins containing SUMO epitope tags
验证活性
1. Western Blot of SUMO protein with Anti-SUMO tag Antibody (5Y461) (TMAS-00131).
Lane 1: 20 ng SUMO protein
Lane 2: 10 ng SUMO protein
Lane 3: 5 ng SUMO protein
Primary Antibody: Anti-SUMO tag Antibody (5Y461) (TMAS-00131), 1 µg/ml.
Secondary Antibody: Peroxidase-AffiniPure Goat Anti-Mouse IgG, Fcγ Fragment Specific, 0.2 μg/ml
Predicted Size: 11 kD
Observed Size: 18 kD
2. Western blot analysis of SUMO-tagged fusion protein using Anti-SUMO tag Antibody (5Y461) (TMAS-00131).
The signal was developed with IRDyeTM800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 62 kD
Observed Size: 62 kD
应用ELISAWB
推荐剂量
ELISA: 0.05-0.2 µg/ml; WB: 0.5-1 µg/ml
抗体种类
Monoclonal
宿主来源Mouse
纯化方式Protein A affinity column
性状Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
缓冲液Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
研究背景SUMO tags are emerging as a viable alternative for increasing both the expression and solubility of otherwise hard-to-express proteins. The SUMO tag can be cleanly excised using SUMO protease, which recognizes the conformation of SUMO protein rather than a specific sequence within SUMO. The SUMO system can be used effectively in both prokaryotic and eukaryotic systems.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: yeast SUMO protein (DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDG IRIQADQAPEDLDMEDNDIIEAHREQIGG)
储存&溶解度
储存方式The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储-20°C for 1 year

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