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Anti-STUB1 Antibody (3D962)
还可以产品编号 TMAY-02372
Anti-STUB1 Antibody (3D962) 是一种 Rabbit 抗体，靶向 STUB1。Anti-STUB1 Antibody (3D962) 可用于 WB,ELISA,FCM,ICC/IF,IP。
Anti-STUB1 Antibody (3D962)
Anti-STUB1 Antibody (3D962)
还可以产品编号 TMAY-02372
Anti-STUB1 Antibody (3D962) 是一种 Rabbit 抗体，靶向 STUB1。Anti-STUB1 Antibody (3D962) 可用于 WB,ELISA,FCM,ICC/IF,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-STUB1 Antibody (3D962) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (3D962) can be used in WB,ELISA,FCM,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3D962 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-STUB1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HELA Whole Cell Lysate. -Lane B: 293T Whole Cell Lysate. -Lane C: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:35 kDa. -Observed band size:36 kDa. 2. STUB1 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate. -Lane C:0.5 mg MCF-7 Whole Cell Lysate. -2 µL anti-STUB1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-STUB1 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 35 kDa. -Observed band size: 35 kDa. 3. Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human STUB1 monoclonal antibody (dilution ratio 1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus. 4. Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | WB,ELISA,FCM,ICC/IF,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:100-1:500; FCM: 1:100-1:500; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human STUB1 isoform 1 (Q9UNE7-1; Met 1-Tyr 303). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | CHIP, the protein encoded by STUB1, is a central component of cellular protein homeostasis and interacts with several key proteins involved in the pathogenesis of manifold neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. CHIP/STUB1 ubiquitin ligase is a negative co-chaperone for HSP90/HSC70, and its expression is reduced or lost in several cancers, including breast cancer. Pathogenic variants in STUB1 were initially described in autosomal recessive spinocerebellar ataxia type 16 and dominant cerebellar ataxia with cerebellar cognitive dysfunction (SCA48). Data indicated that STUB1 deficiency leads both to the intracellular accumulation of protein aggregates and to an increase in the secretion of small extracellular vesicles (sEVs), including exosomes. Secreted sEVs are enriched in ubiquitinated and/or undegraded proteins and protein oligomers. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human STUB1 protein |
| 抗原种属 | Human |
| 研究领域 | Adaptor Proteins |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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