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Anti-Strep Tag II Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Strep Tag II。Anti-Strep Tag II Polyclonal Antibody 可用于 WB。
Anti-Strep Tag II Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Strep Tag II。Anti-Strep Tag II Polyclonal Antibody 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-Strep Tag II Polyclonal Antibody is a Rabbit antibody targeting Strep Tag II. Anti-Strep Tag II Polyclonal Antibody can be used in WB. |
别名 | Strep-Tag-II, Strep.Tag, Strep Tag II, Strep |
Ig Type | IgG |
交叉反应 | Species independent |
验证活性 | 1. Sample: Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysate at 2 μg Lane 2: Strep-Tagged Fusion Protein Overexpression E.coli Lysate at 4 μg Primary: Anti-Strep-Tag II (TMAB-01795) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa 2. Sample: Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysate Lane 2: Negative control Primary: Anti-Strep-Tag II (TMAB-01795) at 1/10000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
浓度 | 1 mg/mL |
研究背景 | The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. |
免疫原 | KLH conjugated Strep-Tag |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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