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Anti-SRSF1 Antibody (4J752)
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产品编号 TMAC-03715
别名 SRSF1, Splicing factor, arginine/serine-rich 1, SFRS1, SF2P33, SF2, Serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, ASF, Alternative-splicing factor 1 (ASF-1)
Anti-SRSF1 Antibody (4J752) 是一种 Rabbit 抗体，靶向 SRSF1。Anti-SRSF1 Antibody (4J752) 可用于 WB,ICC,IF,IHC,FCM。
Anti-SRSF1 Antibody (4J752)
Anti-SRSF1 Antibody (4J752)
还可以产品编号 TMAC-03715 别名 SRSF1, Splicing factor, arginine/serine-rich 1, SFRS1, SF2P33, SF2, Serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, ASF, Alternative-splicing factor 1 (ASF-1)
Anti-SRSF1 Antibody (4J752) 是一种 Rabbit 抗体，靶向 SRSF1。Anti-SRSF1 Antibody (4J752) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SRSF1 Antibody (4J752) is a Rabbit antibody targeting SRSF1. Anti-SRSF1 Antibody (4J752) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | SRSF1, Splicing factor, arginine/serine-rich 1, SFRS1, SF2P33, SF2, Serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, ASF, Alternative-splicing factor 1 (ASF-1) |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart, Lane 2: Mouse liver, Lane 3: K562. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-SF2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SF2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SF2 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SF2 antibody. Counter stained with hematoxylin. 7. ICC staining SF2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of K562 cells with SF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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