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Anti-SPARCL1 Antibody (5W986)
还可以产品编号 TMAY-00163
Anti-SPARCL1 Antibody (5W986) 是一种 Mouse 抗体，靶向 SPARCL1。Anti-SPARCL1 Antibody (5W986) 可用于 ELISA(Det),FCM,ICC/IF。
Anti-SPARCL1 Antibody (5W986)
Anti-SPARCL1 Antibody (5W986)
还可以产品编号 TMAY-00163
Anti-SPARCL1 Antibody (5W986) 是一种 Mouse 抗体，靶向 SPARCL1。Anti-SPARCL1 Antibody (5W986) 可用于 ELISA(Det),FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SPARCL1 Antibody (5W986) is a Mouse antibody targeting SPARCL1. Anti-SPARCL1 Antibody (5W986) can be used in ELISA(Det),FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 5W986 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human SPARCL1 in A431 or Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human SPARCL1 expression on HL60 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human SPARCL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA(Det),FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SPARCL1 / SPARC-like 1 (rh SPARCL1 / SPARC-like 1; NP_004675.3Met1-Phe664). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. Besides, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS). |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Human SPARCL1 / SPARC-like 1 protein |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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