Anti-SNAIL + SLUG Polyclonal Antibody is a Rabbit antibody targeting SNAIL + SLUG. Anti-SNAIL + SLUG Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig,Danio rerio)

1. Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C).
Primary Antibody (green line): Rabbit Anti-SNAIL + SLUG antibody (TMAB-01747),
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-SNAIL+SLUG antibody (TMAB-01747)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Heart Cell (mouse) Lysate at 40 μg
Primary: Anti-SNAIL + SLUG (TMAB-01747) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
4. Sample:
Heart Cell (Rat) Lysate at 40 μg
Primary: Anti-SNAIL + SLUG (TMAB-01747) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
5. Sample:
H9C2 Cell (Rat) Lysate at 30 μg
HUVEC Cell (Human) Lysate at 30 μg
MAD-MB-231 Cell (Human) Lysate at 30 μg
293T Cell (Human) Lysate at 30 μg
Primary: Anti-SNAIL + SLUG (TMAB-01747) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human SNAIL + SLUG

Human