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Anti-SMYD3 Antibody (2M615) 是一种 Rabbit 抗体,靶向 SMYD3。Anti-SMYD3 Antibody (2M615) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-SMYD3 Antibody (2M615) 是一种 Rabbit 抗体,靶向 SMYD3。Anti-SMYD3 Antibody (2M615) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-SMYD3 Antibody (2M615) is a Rabbit antibody targeting SMYD3. Anti-SMYD3 Antibody (2M615) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | ZNFN3A1, ZMYND1, SET and MYND domain containing 3, KMT3E, bA74P14.1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution. Positive control: Lane1: Rat kidney, Lane2: PC-12, Lane3: Hela, Lane4: Mouse spleen, Lane5: NIH-3T3, Lane6: Rat spleen. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMYD3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMYD3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SMYD3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin. 6. ICC staining SMYD3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining SMYD3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with SMYD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain. Histanes methyltransferase. Methylans 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC- 3 'or 5'-GAGGGG-3'. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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