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Anti-SMC3 Antibody (2D413)
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产品编号 TMAC-03806
别名 wu:fc30d07, u:fb22e01, Structural maintenance of chromosomes protein 3, Structural maintenance of chromosomes 3, Structural maintenance of chromosome 3, SMC3L1, SMC3_HUMAN, SMC-3, SMC protein 3, SMC 3, im:7142991, Human chromosome associated polypeptide, hCAP, CSPG6, CSPG 6, Chromosome-associated polypeptide, Chromosome associated polypeptide, chondroitin sulfate proteoglycan 6 (bamacan), Chondroitin sulfate proteoglycan 6, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Basement membrane associated chondroitin proteoglycan, Bamacan, BAM
Anti-SMC3 Antibody (2D413) 是一种 Rabbit 抗体，靶向 SMC3。Anti-SMC3 Antibody (2D413) 可用于 WB,ICC/IF,IHC,FCM。
Anti-SMC3 Antibody (2D413)
Anti-SMC3 Antibody (2D413)
还可以产品编号 TMAC-03806 别名 wu:fc30d07, u:fb22e01, Structural maintenance of chromosomes protein 3, Structural maintenance of chromosomes 3, Structural maintenance of chromosome 3, SMC3L1, SMC3_HUMAN, SMC-3, SMC protein 3, SMC 3, im:7142991, Human chromosome associated polypeptide, hCAP, CSPG6, CSPG 6, Chromosome-associated polypeptide, Chromosome associated polypeptide, chondroitin sulfate proteoglycan 6 (bamacan), Chondroitin sulfate proteoglycan 6, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Basement membrane associated chondroitin proteoglycan, Bamacan, BAM
Anti-SMC3 Antibody (2D413) 是一种 Rabbit 抗体，靶向 SMC3。Anti-SMC3 Antibody (2D413) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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| 产品描述 | Anti-SMC3 Antibody (2D413) is a Rabbit antibody targeting SMC3. Anti-SMC3 Antibody (2D413) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | wu:fc30d07, u:fb22e01, Structural maintenance of chromosomes protein 3, Structural maintenance of chromosomes 3, Structural maintenance of chromosome 3, SMC3L1, SMC3_HUMAN, SMC-3, SMC protein 3, SMC 3, im:7142991, Human chromosome associated polypeptide, hCAP, CSPG6, CSPG 6, Chromosome-associated polypeptide, Chromosome associated polypeptide, chondroitin sulfate proteoglycan 6 (bamacan), Chondroitin sulfate proteoglycan 6, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Basement membrane associated chondroitin proteoglycan, Bamacan, BAM |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution. Positive control: Line1: HepG2, Line2: NIH-3T3, Line3:PC12. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin. 6. ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with SMC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 141 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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