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Anti-SMAD2 Antibody (4E423)

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Anti-SMAD2 Antibody (4E423)
产品编号 TMAC-03786
别名 SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8

Anti-SMAD2 Antibody (4E423) 是一种 Rabbit 抗体,靶向 SMAD2。Anti-SMAD2 Antibody (4E423) 可用于 WB,ICC,IHC,IP,FCM。

Anti-SMAD2 Antibody (4E423)

Anti-SMAD2 Antibody (4E423)

Rating icon 还可以
Anti-SMAD2 Antibody (4E423)
产品编号 TMAC-03786 别名 SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8

Anti-SMAD2 Antibody (4E423) 是一种 Rabbit 抗体,靶向 SMAD2。Anti-SMAD2 Antibody (4E423) 可用于 WB,ICC,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-SMAD2 Antibody (4E423) is a Rabbit antibody targeting SMAD2. Anti-SMAD2 Antibody (4E423) can be used in WB,ICC,IHC,IP,FCM.
别名SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: A431, Lane 3: PC12.
2. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Smad2 antibody. Counter stained with hematoxylin.
3. ICC staining Smad2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Smad2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Smad2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of NIH/3T3 cells with Smad2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 58 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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