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别名 Solute carrier family 1 member 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/aspartate transporter II, GLT1, Excitatory amino acid transporter 2, EAAT2
Anti-SLC1A2 Antibody (9S258) 是一种抗体,靶向 SLC1A2。Anti-SLC1A2 Antibody (9S258) 可用于 ELISA,FCM,WB。
Anti-SLC1A2 Antibody (9S258) 是一种抗体,靶向 SLC1A2。Anti-SLC1A2 Antibody (9S258) 可用于 ELISA,FCM,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
| 产品描述 | Anti-SLC1A2 Antibody (9S258) is an antibody targeting SLC1A2. Anti-SLC1A2 Antibody (9S258) can be used in ELISA,FCM,WB. |
| 别名 | Solute carrier family 1 member 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/aspartate transporter II, GLT1, Excitatory amino acid transporter 2, EAAT2 |
| Ig Type | Rabbit IgG |
| 克隆号 | 9S258 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: U251 whole cell lysate -All lanes: EAAT2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62 kDa -Observed band size: 62 kDa 2. Overlay Peak curve showing MCF-7 cells surface stained with TMAH-01098 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISAFCMWB |
| 推荐剂量 | WB:1:500-1:2000; FCM:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human SLC1A2 |
| 抗原种属 | Human |
| 基因名称 | SLC1A2 |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Neuroscience, Metabolism, Signal transduction |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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