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Anti-SHH Antibody (8Q785)
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产品编号 TMAC-03822
别名 sonic hedgehog, M100081, Hxl3, Hx, Hhg1, Dsh, 9530036O11Rik
Anti-SHH Antibody (8Q785) 是一种 Rabbit 抗体，靶向 SHH。Anti-SHH Antibody (8Q785) 可用于 WB,ICC/IF,IHC,FCM。
Anti-SHH Antibody (8Q785)
Anti-SHH Antibody (8Q785)
还可以产品编号 TMAC-03822 别名 sonic hedgehog, M100081, Hxl3, Hx, Hhg1, Dsh, 9530036O11Rik
Anti-SHH Antibody (8Q785) 是一种 Rabbit 抗体，靶向 SHH。Anti-SHH Antibody (8Q785) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-SHH Antibody (8Q785) is a Rabbit antibody targeting SHH. Anti-SHH Antibody (8Q785) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | sonic hedgehog, M100081, Hxl3, Hx, Hhg1, Dsh, 9530036O11Rik |
| Ig Type | IgG |
| 克隆号 | 8Q785 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin. 4. ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:200-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The Drosophila segment polarity gene hedgehog (hh) encodes a precursor protein which undergoes autocleavage to generate amino- and carboxy-terminal peptides. Both proteins are secreted and appear to function in embryonic and imaginal disc patterning. Several vertebrate homologs of Drosophila hh have been identified. These include Sonic hedgehog (Shh) (alternatively designated Vhh-1), Desert hedgehog (Dhh) and Indian hedgehog (Ihh). Each contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Shh resembles Drosophila hh in that it is processed to generate an amino-terminal secreted peptide that is retained at or near the cell surface and a carboxy-terminal glycosylated more diffusible peptide. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 50 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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