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Anti-SDHB Antibody (3I144)
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产品编号 TMAC-03698
别名 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, SDH1, SDH, mitochondrial, Iron-sulfur subunit of complex II, Ip, EC 1.3.5.1
Anti-SDHB Antibody (3I144) 是一种 Rabbit 抗体，靶向 SDHB。Anti-SDHB Antibody (3I144) 可用于 WB,IHC,FCM,IP。
Anti-SDHB Antibody (3I144)
Anti-SDHB Antibody (3I144)
还可以产品编号 TMAC-03698 别名 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, SDH1, SDH, mitochondrial, Iron-sulfur subunit of complex II, Ip, EC 1.3.5.1
Anti-SDHB Antibody (3I144) 是一种 Rabbit 抗体，靶向 SDHB。Anti-SDHB Antibody (3I144) 可用于 WB,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-SDHB Antibody (3I144) is a Rabbit antibody targeting SDHB. Anti-SDHB Antibody (3I144) can be used in WB,IHC,FCM,IP. |
| 别名 | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, SDH1, SDH, mitochondrial, Iron-sulfur subunit of complex II, Ip, EC 1.3.5.1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/500 dilution. Positive control: Lane 1: Human liver, Lane 2: Rat spleen, Lane 3: Rat liver, Lane 4: Mouse spleen, Lane 5: HepG2. 2. Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-SDHB antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SDHB antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SDHB antibody. Counter stained with hematoxylin. 7. Flow cytometric analysis of Jurkat cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 32 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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