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Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627)
还可以产品编号 TMAY-02883
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627) 是一种 Chimera 抗体，靶向 SARS-CoV/SARS-CoV-2 Spike。Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627) 可用于 ELISA,FCM,ICC/IF。
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627)
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627)
还可以产品编号 TMAY-02883
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627) 是一种 Chimera 抗体，靶向 SARS-CoV/SARS-CoV-2 Spike。Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627) 可用于 ELISA,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Spike. Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3Q627) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal mouse (variable region) / human (kappa / IgG1 constant) chimeric antibody |
| 克隆号 | 3Q627 |
| 验证活性 | 1. Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV Spike monoclonal antibody (dilution ratio 1:60) at 37°C for 1 hour. Then cells were stained with the FITC-conjugated Goat Anti-human IgG secondary antibody (green). (Validation Experiment) 2. Immunofluorescence analysis of Spike overexpressed Hek293 Cells were stained with purified anti-SARS Spike Chimera Mab,then a FITC-conjugated second step antibody. (Validation Experiment) 3. Flow cytometric analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Spike Chimeric MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. (Validation Experiment) |
| 应用 | ELISA,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Chimera |
| 构建方式 | It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Biotin/HRP |
| 可提供抗体种类 | 3 |
| 免疫原 | Recombinant SARS-CoV Spike RBD Protein (TMPY-03224) |
| 抗原种属 | SARS |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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