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Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)

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Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)
产品编号 TMAY-02970

Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) 是一种 Rabbit 抗体，靶向 SARS-CoV-2 Nucleocapsid。Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) 可用于 WB,ELISA,ELISA(Det),FCM,ICC/IF,IP。

Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)
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Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)

Rating icon 还可以
Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259)
产品编号 TMAY-02970

Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) 是一种 Rabbit 抗体，靶向 SARS-CoV-2 Nucleocapsid。Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) 可用于 WB,ELISA,ELISA(Det),FCM,ICC/IF,IP。

规格价格库存数量
20 μL
¥ 1,995
5日内发货
100 μL
¥ 3,995
5日内发货
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产品介绍

生物活性
产品描述
Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) is a Rabbit antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (2G259) can be used in WB,ELISA,ELISA(Det),FCM,ICC/IF,IP.
Ig Type
Rabbit IgG
克隆号
2G259
验证活性
1. Flow cytometric analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid Rabbit MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. (Validation Experiment)
2. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid rabbit monoclonal antibody at 1:5000 dilution.
-Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate.
-Lane B: HEK293 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions. (Validation Experiment)
3. Immunofluorescence analysis of SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Cells were stained with purified anti-SARS-CoV-2 Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody. (Validation Experiment)
4. SARS-COV-2 Nucleocapsid was immunoprecipitated using:
-Lane A:0.5 mg SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate.
-Lane B:0.5 mg HEK293 Whole Cell Lysate.
-4 µL anti-SARS-COV-2 Nucleocapsid rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-anti-SARS-COV-2 Nucleocapsid rabbit polyclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions. (Validation Experiment)
应用
WB,ELISA,ELISA(Det),FCM,ICC/IF,IP
推荐剂量
WB: 1:5000-1:20000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; IP: 1-5 µL/mg of lysate; ELISA(Det): 1:1000-1:10000
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This product is a recombinant monoclonal antibody expressed from HEK293 cells.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (TMPY-05664)
抗原种属
SARS-CoV-2
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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