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Anti-S100A9 Antibody (7L821)
还可以产品编号 TMAY-01695
别名 S100 calcium binding protein A9, p14, MRP14, L1Ag, GAGB, Cagb, BEE22, AW546964, 60B8Ag
Anti-S100A9 Antibody (7L821) 是一种 Mouse 抗体，靶向 S100A9。Anti-S100A9 Antibody (7L821) 可用于 FCM,ICC/IF。
Anti-S100A9 Antibody (7L821)
Anti-S100A9 Antibody (7L821)
还可以产品编号 TMAY-01695 别名 S100 calcium binding protein A9, p14, MRP14, L1Ag, GAGB, Cagb, BEE22, AW546964, 60B8Ag
Anti-S100A9 Antibody (7L821) 是一种 Mouse 抗体，靶向 S100A9。Anti-S100A9 Antibody (7L821) 可用于 FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,684 | 5日内发货 |
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偶联与修饰
抗原信息
| 产品描述 | Anti-S100A9 Antibody (7L821) is a Mouse antibody targeting S100A9. Anti-S100A9 Antibody (7L821) can be used in FCM,ICC/IF. |
| 别名 | S100 calcium binding protein A9, p14, MRP14, L1Ag, GAGB, Cagb, BEE22, AW546964, 60B8Ag |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 7L821 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human S100A9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human S100A9 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus. 2. Flow cytometric analysis of Human S100A9 on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human S100A9 (Filled hisgram), then stained with a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by Anti-S100A9 Antibody was blocked by preincubation of the purified antibody with 20ug recombinant human S100A9 for 1 hour (Black solid line hisgram). |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A9 (rh S100A9; TMPY-01357; NP_002956.1; Met1-Pro114). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calciumbinding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC/PE |
| 可提供抗体种类 | 3 |
| 免疫原 | Recombinant Human S100A9 protein (TMPY-01357) |
| 抗原种属 | Human |
| 研究领域 | Calcium-binding Proteins and Related Molecules |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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