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Anti-RND3 Antibody (2F966) 是一种 Mouse 抗体,靶向 RND3。Anti-RND3 Antibody (2F966) 可用于 WB,ICC,IHC,FCM。
Anti-RND3 Antibody (2F966) 是一种 Mouse 抗体,靶向 RND3。Anti-RND3 Antibody (2F966) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,990 | 5日内发货 | |
100 μL | ¥ 2,990 | 5日内发货 |
产品描述 | Anti-RND3 Antibody (2F966) is a Mouse antibody targeting RND3. Anti-RND3 Antibody (2F966) can be used in WB,ICC,IHC,FCM. |
别名 | Small GTP binding protein Rho8, RND3_HUMAN, Rnd3, RND 3, Rho-related GTP-binding protein RhoE, Rho-related GTP-binding protein Rho8, RhoE, Rho8, Rho family GTPase 3, Ras homolog gene family member E, Protein MemB, memB, ARHE |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of RND3 on human RND3 recombinant protein using anti-RND3 antibody at 1/1,000 dilution. 2. Western blot analysis of RND3 on HEK293 (1) and RND3-hIgGFc transfected HEK293 (2) cell lysate using anti-RND3 antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RND3 antibody. Counter stained with hematoxylin. 4. ICC staining RND3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:100-200 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 27 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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