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Anti-RING1 Antibody (1C89)
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产品编号 TMAC-03607
别名 Transcription repressor Ring1A, Rnf1, RING1A, Ring1A, RING1_HUMAN, RING finger protein 1, Ring 1, Really interesting new gene 1 protein, Polycomb complex protein RING1, E3 ubiquitin-protein ligase RING1
Anti-RING1 Antibody (1C89) 是一种 Mouse 抗体，靶向 RING1。Anti-RING1 Antibody (1C89) 可用于 WB,IHC,FCM。
Anti-RING1 Antibody (1C89)
Anti-RING1 Antibody (1C89)
还可以产品编号 TMAC-03607 别名 Transcription repressor Ring1A, Rnf1, RING1A, Ring1A, RING1_HUMAN, RING finger protein 1, Ring 1, Really interesting new gene 1 protein, Polycomb complex protein RING1, E3 ubiquitin-protein ligase RING1
Anti-RING1 Antibody (1C89) 是一种 Mouse 抗体，靶向 RING1。Anti-RING1 Antibody (1C89) 可用于 WB,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,980 | 5日内发货 | |
| 100 μL | ¥ 2,980 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-RING1 Antibody (1C89) is a Mouse antibody targeting RING1. Anti-RING1 Antibody (1C89) can be used in WB,IHC,FCM. |
| 别名 | Transcription repressor Ring1A, Rnf1, RING1A, Ring1A, RING1_HUMAN, RING finger protein 1, Ring 1, Really interesting new gene 1 protein, Polycomb complex protein RING1, E3 ubiquitin-protein ligase RING1 |
| 克隆号 | 1C89 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Ring1 on human Ring1 recombinant protein using anti-Ring1 antibody at 1/1,000 dilution. 2. Western blot analysis of Ring1 on HEK293 (1) and Ring1-hIgGFc transfected HEK293 (2) cell lysate using anti-Ring1 antibody at 1/1,000 dilution. 3. Western blot analysis of Ring1 on different cell lysate using anti-Ring1 antibody at 1/1,000 dilution. Positive control: Line1: MOLT-4, Line2: LNCaP, Line3: Hela, Line4: HEK-293, Line5: Jurkat. 4. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of Hela cells with Ring1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:100-200; FCM: 1:100-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci during embryonic development. The human PcG homologues form two distinct multimeric protein complexes, the EED/EZH protein complex and the HPC/HPH protein complex, which have mutually exclusive expression patterns. The HPC/HPH PcG complex contains the human polycomb 2 (HPC2), human polyhomeotic (HPH), Bmi-1 and RING1 proteins. The human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six, encodes for a protein that contains a RING finger motif, a zinc-binding domain found in many regulatory proteins, but unlike the other human PcG genes, RING1 displays no homology to known Drosophila PcG genes. RING1 strongly represses En-2, the mammalian homolog of the Drosophila engrailed gene, and when overexpressed, it mediates an increase in the expression of proto-oncogenes, such as c-Jun and c-fos. Also, loss of RING1 and Bmi-1 expression correlates with the differentiation of B cells, which suggests a role for RING1 in germinal center development. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 50 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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