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Anti-RhoA/B/C Antibody (1S695) 是一种 Rabbit 抗体,靶向 RhoA/B/C。Anti-RhoA/B/C Antibody (1S695) 可用于 WB,ICC/IF,IHC,FCM。
Anti-RhoA/B/C Antibody (1S695) 是一种 Rabbit 抗体,靶向 RhoA/B/C。Anti-RhoA/B/C Antibody (1S695) 可用于 WB,ICC/IF,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-RhoA/B/C Antibody (1S695) is a Rabbit antibody targeting RhoA/B/C. Anti-RhoA/B/C Antibody (1S695) can be used in WB,ICC/IF,IHC,FCM. |
别名 | Transforming protein RhoA, Small GTP binding protein RhoC, small GTP binding protein RhoA, RP11-426L16.4, RHOH9, RHOH6, RHOH12, rhoC GTPase, RHOC, RHOB, RHOA, RHO12, Rho related GTP binding protein RhoC, Rho related GTP binding protein RhoB, rho cDNA clone 9, rho cDNA clone 6, Rho cDNA clone 12, Rho A/B/C, Rh -related GTP binding protein RhoC, RAS-related homolog 9, Ras homolog gene family, member C, Ras homolog gene family, member B, Ras homolog gene family, member A, Ras homolog 9 (RhoC), OTTMUSP00000025087, OTTMUSP00000025086, OTTHUMP00000013809, OTTHUMP00000013807, OTTHUMP00000013805, OTTHUMP00000013802, OTTHUMP00000013676, OTTHUMP00000013675, Oncogene RHO H9, Oncogene RHO H6, Oncogene RHO H12, MSTP081, MST081, MGC61427, MGC1448, MGC117867, H9, H6, H12, ARHC, ARHB, ARHA, ARH9, ARH6, ARH12, Aplysia RAS-related homolog 6, Aplysia ras-related homolog 12, Aplysia RAS-related homolog, Aplysia ras related homolog 9 (RhoC), AI324259,, AA017882 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Hela, Lane 3: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin. 5. ICC staining Rho A+B+C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Rho A+B+C in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Rho A+B+C in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of HepG2 cells with Rho A+B+C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:200-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
分子量 | Theoretical: 24 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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