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Anti-RhoA Antibody (1W191)
还可以产品编号 TMAY-02348
Anti-RhoA Antibody (1W191) 是一种 Mouse 抗体，靶向 RhoA。Anti-RhoA Antibody (1W191) 可用于 ELISA,FCM,ICC/IF。
Anti-RhoA Antibody (1W191)
Anti-RhoA Antibody (1W191)
还可以产品编号 TMAY-02348
Anti-RhoA Antibody (1W191) 是一种 Mouse 抗体，靶向 RhoA。Anti-RhoA Antibody (1W191) 可用于 ELISA,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-RhoA Antibody (1W191) is a Mouse antibody targeting RhoA. Anti-RhoA Antibody (1W191) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 1W191 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human RHOA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human RHOA monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human RHOA expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-RHOA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RhoA (rh RhoA; TMPY-01958; P61586-1; Met 1-Leu 193). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein that belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | PE |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Human RhoA protein (TMPY-01958) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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