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Anti-RBP4 Antibody (2R164)
还可以产品编号 TMAY-03155
Anti-RBP4 Antibody (2R164) 是一种 Rabbit 抗体，靶向 RBP4。Anti-RBP4 Antibody (2R164) 可用于 FCM,ICC/IF。
Anti-RBP4 Antibody (2R164)
Anti-RBP4 Antibody (2R164)
还可以产品编号 TMAY-03155
Anti-RBP4 Antibody (2R164) 是一种 Rabbit 抗体，靶向 RBP4。Anti-RBP4 Antibody (2R164) 可用于 FCM,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-RBP4 Antibody (2R164) is a Rabbit antibody targeting RBP4. Anti-RBP4 Antibody (2R164) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 2R164 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Flow cytometric analysis of Mouse RBP4 expression on BABL/c splenocytes. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Mouse RBP4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of mouse RBP4 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse RBP4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse RBP4 (rM RBP4; TMPY-01567; NP_035385.1; Met1-Leu201). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Mouse RBP4 protein (TMPY-01567) |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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